Publications 2013 - 2017


  • Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions
    Vibhute, M. A., Schaap, M. H., Maas, R. J. M., Nelissen, F. H. T., Spruijt, E., Heus, H. A., Hansen, M. M. K. and Huck, W. T. S. (2020).
    Acs Synthetic Biology, 9 (10), 2797-2807. 10.1021/acssynbio.0c00330

  • Ramified rolling circle amplification for synthesis of nucleosomal DNA sequences
    van Emmerik, C. L., Gachulincova, I., Lobbia, V. R., Daniels, M. A., Heus, H. A., Soufi, A., Nelissen, F. H. T. and van Ingen, H. (2020).
    Analytical Biochemistry, 588. 10.1016/j.ab.2019.113469

  • Proximity-induced caspase-9 activation on a DNA origami-based synthetic apoptosome
    Rosier, B. J. H. M., Markvoort, A. J., Audenis, B. G., Roodhuizen, J. A. L., den Hamer, A., Brunsveld, L. and de Greef, T. F. A. (2020).
    Nature Catalysis, 3 (3), 295-306.  10.1038/s41929-019-0403-7

  • Designed Asymmetric Protein Assembly on a Symmetric Scaffold
    Lemmens, L. J. M., Roodhuizen, J. A. L., de Greef, T. F. A., Markvoort, A. J. and Brunsveld, L. (2020).
    Angewandte Chemie-International Edition, 59 (29), 12113-12121. 10.1002/anie.202003626

  • Engineered Living Materials Based on Adhesin-Mediated Trapping of Programmable Cells
    Guo, S. Q., Dubuc, E., Rave, Y., Verhagen, M., Twisk, S. A. E., van der Hek, T., Oerlemans, G. J. M., van den Oetelaar, M. C. M., van Hazendonk, L. S., Bruls, M., Eijkens, B. V., Joostens, P. L., Keij, S. R., Xing, W. Z., Nijs, M., Stalpers, J., Sharma, M., Gerth, M., Boonen, R. J. E. A., Verduin, K., Merkx, M., Voets, I. K. and de Greef, T. F. A. (2020). 
    Acs Synthetic Biology, 9 (3), 475-485. 10.1021/acssynbio.9b00404

  • Dynamic modulation of proximity-induced enzyme activity using supramolecular polymers
    Estirado, E. M., Rosier, B. J. H. M., de Greef, T. F. A. and Brunsveld, L. (2020). 
    Chemical Communications, 56 (43), 5747-5750. 10.1039/d0cc02120b


  • A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
    van der Linden, A. J., Yelleswarapu, M., Pieters, P., Swank, Z., Huck, W. T. S., Maerkl, S. J. and de Greef, T. F. A. (2019). 
    Jove-Journal of Visualized Experiments, 152. ARTN e59655

  • Supramolecular interactions between catalytic species allow rational control over reaction kinetics
    Teunissen, A. J. P., Paffen, T. F. E., Filot, I. A. W., Lanting, M. D., van der Haas, R. J. C., de Greef, T. F. A. and Meijer, E. W. (2019).
    Chemical Science, 10 (39), 9115-9124. 10.1039/c9sc02357g

  • Counterion-Dependent Mechanisms of DNA Origami Nanostructure Stabilization Revealed by Atomistic Molecular Simulation
    Roodhuizen, J. A. L., Hendrikx, P. J. T. M., Hilbers, P. A. J., de Greef, T. F. A. and Markvoort, A. J. (2019).
    Acs Nano, 13 (9), 10798-10809. 10.1021/acsnano.9b05650

  • DNA-based communication in populations of synthetic protocells
    Joesaar, A., Yang, S., Bogels, B., van der Linden, A., Pieters, P., Kumar, B. V. V. S. P., Dalchau, N., Phillips, A., Mann, S. and de Greef, T. F. A. (2019).
    Nature Nanotechnology, 14 (4), 369-+. 10.1038/s41565-019-0399-9

  • Pathway Control in Cooperative vs. Anti-Cooperative Supramolecular Polymers
    Herkert, L., Droste, J., Kartha, K. K., Korevaar, P. A., de Greef, T. F. A., Hansen, M. R. and Fernandez, G. (2019).
    Angewandte Chemie-International Edition, 58 (33), 11344-11349.

  • Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks
    Dubuc, E., Pieters, P. A., van der Linden, A. J., van Hest, J. C. M., Huck, W. T. S. and de Greef, T. F. A. (2019).
    Current Opinion in Biotechnology, 58, 72 - 80. 10.1016/j.copbio.2018.10.006

  • Efficient Small-Scale Conjugation of DNA to Primary Antibodies for Multiplexed Cellular Targeting
    Cremers, G. A. O., Rosier, B. J. H. M., Brillas, R. R., Albertazzi, L. and de Greef, T. F. A. (2019).
    Bioconjugate Chemistry, 30 (9), 384-2392. 10.1021/acs.bioconjchem.9b00490


  • Trace analysis in water-alcohol mixtures by continuous p-H-2 hyperpolarization at high magnetic field
    Hermkens, N.K.J., Aspers, R.L.E.G. , Feiters, M.C., Rutjes, F.P.J.T.  & Tessari, M. (2018).
    Magnetic Resonance in Chemistry, 56 (7), 633-640. doi: 10.1002/mrc.4692

  • Dissipative adaptation in driven self-assembly leading to self-dividing fibrils
    te Brinke E., Groen J., Herrmann A., Heus H. A., Rivas G., Spruijt E. & Huck W. T. S. (2018).  Nature nanotechnology, 13, 849-+.


  • Biomimetic stress sensitive hydrogel controlled by DNA nanoswitches 
    S.R. Deshpande, R. Hammink, F.T.H. Nelissen, A.E. Rowan, and H.A. Heus
    Biomacromolecules (2017), 18, 3310-17. DOI: 10.1021/acs.biomac.7b00964

  • A membrane-anchored aptamer sensor for probing of IFNγ secretion by single cells
    L. Qiu, F.Wimmers, J. Weijden, H.A. Heus, J. Tel, and C.G. Figdor
    Chem. Comm. (2017), 53, 8066 - 69. DOI: 10.1039/c7cc03576d


  • DNA-responsive polyisocyanopeptide hydrogels with stress-stiffening capacity
    S.R. Deshpande, R. Hammink, R.K. Das,  F. H. T. Nelissen , K.G. Blank, A.E. Rowan, and H.A. Heus
    Adv Funct Mat (2016), 26, 9075-82. DOI: 10.1002/adfm.201602461
  • Protein Synthesis in Coupled and Uncoupled Cell-Free Prokaryotic Gene Expression Systems
    M.M. Hansen, M. Ventosa Rosquelles, M.Yelleswarapu, R.J. Maas, A.J. van Vugt-Jonker, H.A.Heus , and W.T.S. Huck 
    ACS Synth Biol  (2016) 5, 1433-1440. DOI: 10.1021/acssynbio.6b00010

  • Cell-Like Nanostructured Environments Alter Diffusion and Reaction Kinetics in Cell-Free Gene Expression.
    M.M. Hansen, S. Paffenholz, D. Foschepoth, H.A. Heus, J. Thiele, and W.T. S. Huck 
    Chembiochem (2016), 17, 228-32. DOI: 10.1002/cbic.201500560

  • Macromolecular crowding creates heterogeneous environments of gene expression in picolitre droplets.
    M. M. Hansen, L. H. Meijer, E. Spruijt, R. J. Maas, M. V. Rosquelles, J. Groen, H. A. Heus, and W. T. S. Huck  
    Nature nanotechnology (2016), 11, 191-7. DOI: 10.1038/NNANO.2015.243

  • Stable isotope labeling methods for DNA 
    F.H.T. Nelissen, M. Tessari, S.S. Wijmenga, and H.A. Heus 
    Prog Nucl Magn Reson Spectrosc (2016), 96, 89-108. DOI: 10.1016/j.pnmrs.2016.06.001
  • Determination of long-range scalar H-H coupling constants responsible for polarization transfer in SABRE
    Eshuis N., Aspers R.L., van Weerdenburg B.J., Feiters M.C., Rutjes F.P., Wijmenga S.S., and Tessari M.
    J Magn Reson(2016), 265, 59-66.
  • NMR-based Chemosensing via p-H2 Hyperpolarization: Application to Natural Extracts.
    Hermkens N.K., Eshuis N., van Weerdenburg B.J., Feiters M.C., Rutjes F.P., Wijmenga S.S., and Tessari M.
    Analytical chemistry (2016). 88, 3406-12.


  • Associative interactions in crowded solutions of biopolymers counteract depletion effects
    J. Groen, D. Foschepot, E. te Brinke, A.J. Boersma, H. Imamura, G. Rivas, H.A. Heus, and W. T. S. Huck  
    J Am Chem Soc (2015), 137, 13041-48. DOI: 10.1021/jacs.5b07898

  • Production of homogeneous recombinant RNA using a tRNA scaffold and hammerhead ribozymes
    F.H.T. Nelissen, H.A Heus, and S.S. Wijmenga  
    Methods Mol Biol. (2015), 1316, 33-44. DOI: 10.1007/978-1-4939-2730-2_4

  • Enzymatic preparation of multimilligram amounts of pure single-stranded DNA samples for material and analytical sciences
    F.H.T. Nelissen, E.P.M. Goossens, M. Tessari, and H.A. Heus   
    Anal Biochem (2015), 475, 68-73. DOI: 10.1016/j.ab.2015.01.014
  • Application of the pi-accepting ability parameter of N-heterocyclic carbene ligands in iridium complexes for signal amplification by reversible exchange (SABRE).
    van Weerdenburg B.J.A., Eshuis N., Tessari M., Rutjes F.P.J.T., and Feiters M.C.  
    Dalton T (2015), 44, 15387-90.

  • Computational (DFT) and Experimental (EXAFS) Study of the Interaction of [Ir(IMes)(H)(2)(L)(3)] with Substrates and Co-substrates Relevant for SABRE in Dilute Systems.
    van Weerdenburg B. J. A., Engwerda A. H. J., Eshuis N., Longo A., Banerjee D., Tessari M., Guerra C. F., Rutjes F. P. J. T., Bickelhaupt F. M., and Feiters M. C.  
    Chem-Eur J (2015), 21, 10482-89.
  • Magnetic Susceptibility to Measure Total Protein Concentration from NMR Metabolite Spectra: Demonstration on Blood Plasma 
    Jupin M., Michiels P. J., Girard F. C., and Wijmenga S. S. 
    Magn Reson Med (2015), 73, 459-68.

  • Quantitative Trace Analysis of Complex Mixtures Using SABRE Hyperpolarization
    Eshuis N., van Weerdenburg B. J. A., Feiters M. C., Rutjes F. P. J. T., Wijmenga S. S., and  Tessari M. 
    Angew Chem Int Edit (2015), 54, 1481-84.

  • 2D NMR Trace Analysis by Continuous Hyperpolarization at High Magnetic Field
    Eshuis N., Aspers R., Weerdenburg B.J.A. van, Feiters M. C., Rutjes F. P. J. T., Wijmenga S. S., and Tessari M. 
    Angew Chem Int Edit (2015), 54, 14527-30


  • DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation.
    J. Thiele, Y. Ma, D. Foschepoth, M.M. Hansen, C. Steffen, H.A. Heus, and W.T.H. Huck 
    Lab  Chip (2014), 14, 2651-56. DOI: 10.1039/c3lc51427g

  • Toward Nanomolar Detection by NMR Through SABRE Hyperpolarization
    Eshuis N, Hermkens N, van Weerdenburg B J, Feiters M C, Rutjes F P, Wijmenga S S, and Tessari M.  
    J Am Chem Soc (2014), 136, 2695-8.
  • Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives from Toad and Cone Snail
    Heus F, Otvos RA, Aspers RL, van Elk R, Halff JI, Ehlers AW, Dutertre S, Lewis RJ, Wijmenga S, Smit AB, Niessen WM, Kool J. (2014) 
    Biology (2014), 3 , 139-156.
  • NMR metabolomics profiling of blood plasma mimics shows that medium- and long-chain fatty acids differently release metabolites from human serum albumin
    M. Jupin, P. J. Michiels, F. C. Girard, M. Spraul and S. S. Wijmenga
    J Magn Reson (2014), 239 , 34-4.
  • How a low-fidelity DNA polymerase chooses non-watson-crick from watson-crick incorporation
    W. J. Wu, M. I. Su, J. L. Wu, S. Kumar, L. H. Lim, C. W. Wang, F. H. Nelissen, M. C. Chen, J. F. Doreleijers, S. S. Wijmenga and M. D. Tsai 
    J Am Chem Soc (2014), 136 , 4927-37

  • Liquid-phase parahydrogen-induced polarization (PHIP) with ligand-capped platinum nanoparticles
    M. Irfan,  Eshuis, N., Spannring, P. , Tessari, M., Feiters, M.C. & Rutjes, F.P.J.T.   
    J Phys Chem C (2014), 118, 13313-19.


  • Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate
    Sokolova, E., Spruijt, E., Hansen, M.M.K., Dubuc, E., Groen, J., Chokkalingam, V., Piruska, A., Heus, H.A., and Huck, W.T.S. 
    Proc Natl Acad Sci U S A (2013), 110, 11692-11697. DOI: 10.1073/pnas.1222321110

  • Structural and thermodynamic signatures that define pseudotriloop RNA hairpins
    van der Werf R., Wijmenga S.S., Heus H.A., and Olsthoorn R.C. 
    RNA (2013), 19 , 1833-9. DOI: 10.1261/rna.039636.113

  • Fluorine detected 2D NMR experiments for the practical determination of size and sign of homonuclear F-F and heteronuclear C-F multiple bond J-coupling constants in multiple fluorinated compounds
    Aspers, R.L.E.G., Arnpt, K.A.M., Dyortsak, P., Jaeger, M. & Wijmenga, S.S. .
    J Magn Reson (2013), 231, 79-89.
  • EC-SPE-stripline-NMR analysis of reactive products: a feasibility study
    Falck, D., Vries, A.J. de, Kolkman, A., Lingeman, H., Honing, M., Wijmenga, S.S., Kentgens, A.P.M. & Niessen, W.M.A.

    Analytical and Bioanalytical Chemistry (2013), 405, 6711-6720.
  • NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction
    Jupin, M., Michiels, P.J., Girard, F.C., Spraul, M. & Wijmenga, S.S.
    J Magn Reson (2013), 228, 81-94.
  • Modification of picornavirus genomic RNA using 'click' chemistry shows that unlinking of the VPg peptide is dispensable for translation and replication of the incoming viral RNA
    Langereis MA, Feng Q, Nelissen FH, Virgen-Slane R, van der Heden van Noort GJ, Maciejewski S, Filippov DV, Semler BL, van Delft FL, and van Kuppeveld FJ
    Nucleic Acids Res (2014), 42, 2473-82.

  • Interconverting Conformations of Slipped-DNA Junctions Formed by Trinucleotide Repeats Affect Repair Outcome
    Slean, M.M., Reddy, K., Wu, B., Edamura, K.N., Kekis, M., Nelissen, F.H.T., Aspers, R., Tessari, M., Scharer, O.D., Wijmenga, S.S., and Pearson, C.E. Biochem (2013), 52, 773-785.
  • Ligand effects of NHC-iridium catalysts for signal amplification by reversible exchange (SABRE)
    van Weerdenburg, B.J.A., Gloggler, S., Eshuis, N., Engwerda, A.H.J., Smits, J.M.M., Gelder, R. de, Appelt, S., Wijmenga, S.S., Tessari, M., Feiters, M.C., Blumich, B., and Rutjes, F.P.J.T.
    Chem Comm (2013), 49, 7388-7390.
  • Nucleic acid helix structure determination from NMR proton chemical shifts
    Werf, R.M. van der, Tessari, M. & Wijmenga S.S. 
    J Biomol NMR (2013), 56, 95-112.