Abstract:
Targeted covalent inhibitors are powerful entities in drug discovery to expand the druggable proteome. Nevertheless, their application has so far mainly been limited to addressing cysteine residues. The development of cysteine-directed covalent inhibitors has largely profited from determining their proteome-wide selectivity using competitive residue-specific proteomics. Several probes have recently been described to monitor other amino acids using this technology and many more electrophiles exist to modify proteins. Nevertheless, a direct, proteome-wide comparison of the selectivity of diverse probes is still entirely missing. Here, we developed a completely unbiased workflow to analyse electrophile selectivity proteome-wide and applied it to directly compare 54 alkyne probes containing diverse reactive groups. In this way, we verified and newly identified probes to monitor a total of nine different amino acids as well as the N terminus proteome-wide. This selection includes the first probes to globally monitor tryptophans, histidines and arginines as well as novel tailored probes for methionines, aspartates and glutamates.
Short C
Dr. Stephan Hacker performed his PhD studies with Prof. Andreas Marx at the University of Konstanz, Germany, and his postdoctoral research with Prof. Benjamin Cravatt at The Scripps Research Institute in La Jolla, USA. Afterwards, he moved to the Technical University of Munich, Germany, to work as an independent group leader. In 2021, he became an Assistant Professor at the Leiden Institute of Chemistry. Stephan Hacker’s group develops chemistries for novel covalent protein ligands targeting diverse amino acids as well as chemoproteomic technologies to study their target engagement with resolution of the modified amino acid residue in proteome-wide studies. His group focuses on the application of these compounds and technologies to identify new druggable target proteins in bacteria.
