Stepping out of the clinic: towards objective, real-life monitoring of Parkinson’s disease

Tuesday 18 June 2024, 10:30 am
PhD candidate
V. Carotti
Promotor(s)
prof. dr. J.G.J. Hoenderop
Co-promotor(s)
dr. J.A.J. van der Wijst
Location
Aula

Extracellular vesicles (EVs) are small particles released by all cells, facilitating intercellular communication by transporting various molecules from cell to cell. Our research demonstrates that EVs released from cells of  the proximal nephron tubule regulate extracellular ATP (eATP) levels in the collecting duct (CT) and potentially influence the expression of the renal channel ENaC. Additionally, EVs from the CT exert biological effects on CT cells themselves. Moreover, we observed increased EV release in various in vitro and in vivo models of Autosomal Dominant Polycystic Kidney Disease (ADPKD), a common genetic disorder linked to renal cyst development. This effect may result from dysregulated lipid ceramide biosynthesis and metabolism. In vitro, we investigated EV involvement in cell-to-cell communication between the distal tubule and the CT in ADPKD models, focusing on pathogenic features such as eATP levels, gene expression, protein phosphorylation, and cyst growth.

Valentina Carotti (1991) obtained her Master’s degree in Medical and Pharmaceutical Biotechnology, cum laude, at the University of Florence in 2017. In 2018, she began her PhD research at the Physiology department of Radboudmc. Currently, she is working as a Research Fellow at the Department of Medical Genetics, Meyer Hospital (Florence, ITA).