Untargeted Plasma Metabolomics of Endocrine Hypertension

Monday 23 January 2023, 10:30 am
Nick Bliziotis

The dissertation was aimed at the discovery of metabolic markers of endocrine hypertension (EHT), which could be used for screening patients with hypertension for a curable form. The initial methodological comparison of Nuclear Magnetic Resonance (NMR) spectroscopy protocols established a workflow to analyze plasma samples. In a subset of the sample cohort, a per-patient comparison of pre-operative samples to their post-operative counterparts, collected from patients with one form of EHT, pheochromocytoma and paraganglioma (PPGL), was carried out. In the entire cohort, EHT samples were separated from controls with high accuracy, but the associated signature was likely compromised by confounding effects of sampling procedures. The application of an alternate method, High-Resolution Mass Spectrometry (HRMS), further investigates these and expands upon the results obtained by NMR. Overall, the thesis underscores the value of considering confounding effects in metabolomics studies.