Recording neuronal activity in 3D using calcium and voltage indicators

Tuesday 9 July 2024, 11 am

We have designed a new optical technique referred as Custom-Address Serial Holography (CASH) that allows recording in 3D in the head-fixed behaving rodent the spiking activity of 20 cells at 1 kHz up to 200 cells at 0.1 kHz across a volume of (500 µm)3 [1]. This is obtained by individual patterning of every laser pulse of a 40 kHz regenerative amplifier using fast acousto-optic modulation [2,3] to serially address the target cells with an extended volume covering the cell body and the surrounding space to prevent motion artefacts [1]. Using GCaMP6f calcium dyes, we analyzed the spatio-temporal organization of hundreds of neuron activity in layer 2/3 and 5 of mouse primary visual cortex in response to moving contrast gratings [1]. Using new genetically-encoded voltage indicators,  we recorded the activity of a few cells in 3D with sub-millisecond precision. Finally, we performed trans-skull imaging over fields of view that extend beyond the scattering memory effect by updating the correction of aberration and scattering at the scanning rate [4]. 

References

[1]         Akemann W, et al. Nat Methods. 19(1):100-110, 2022 

[2]         Akemann W et al. Opt. Express 23(22), 28191-28205, 2015

[3]         Akemann W. and Bourdieu L., APL photonics, 9(4), 046103, 2024. 

[4]         Blochet et al PNAS 120(51), e2305593120.

When
Tuesday 9 July 2024, 11 am
Location
Huygens building HG 00.303