What is the topic of your PhD project and how does your work look like in practice?
When we learn something, our neuronal connections adapt in order to store this information. Locally, the balance between excitatory and inhibitory inputs in the brain will therefore temporarily shift to allow more excitation. Research has shown that inhibitory synapses play a crucial role in controlling synaptic plasticity. Therefore, my research is focused on investigating which molecular mechanisms are involved in the removal of inhibitory synapses.
So how does this look like in practice? We take mice that contain GFP-labeled inhibitory neurons. Next, we dissect and make slices from the hippocampus, a brain region known for its plasticity. We then culture these slices for up to three weeks, in which these neurons retain their physiological properties. By making use of a two-photon microscope, it allows us to follow the dynamic inhibitory synapses over time. Next, we can combine this technique with other cool approaches to alter specific proteins or pathways in our hippocampal slices and track whether this results in the formation, strengthening, loss or weakening of inhibitory synapses.
What aspect of your job do you excel at?
I think patience is very important when doing a PhD and I believe it is one of my more useful traits. The techniques used in my research are not easy and often require trouble-shooting. With curiosity as big driver, it can be frustrating if experiments do not work out as expected. However, it is best to stay calm and begin with structured problem-solving.
What is your favorite book and why?
My favorite book is The Power of Habit by Charles Duhigg. In this book, the underlying science of habits are explained by making use of unique examples of history. The author breaks down the different aspects of habits and how new habits were introduced in the past. These examples range from toothpaste and cleaning sprays to how Rosa Parks’ actions resulted in the Montgomery Bus Boycott but not when another black person refused to give up their seat. This books motivates to change unwanted habits and offers tools to do so.