A.A.M. André (Alain)
Employee - Physical Organic Chemistry
6525 AJ NIJMEGEN
Internal postal code: 50
6500 GL NIJMEGEN
Biomolecular condensates, or membraneless organelles are dense liquid droplets, enriched with proteins and nucleic acids. These droplets arise from a process called liquid-liquid phase separation (LLPS), or coacervation. The proteins enriched in these droplets are typically intrinsically disordered and enriched with charged residues, linking them to polyelectrolytes. By mimicking these condensates in a cell-free environment, we aim to better understand the molecular mechanism and principles of LLPS in cells. What are the advantages and disadvantages of condensation?
Alain André received his M.Sc. in molecular life sciences from Radboud University in 2017. During his studies he worked on photo-switchable cell penetrating peptides in the Bio-Organic Chemistry group of Prof. Jan van Hest (Radboud University), biohybrid stomatocyte nanomotors for drug delivery in the group of Systems Chemistry of Prof. Daniela Wilson (Radboud University) and on structural analysis of the motif-B domain of BET-proteins at the Protein Structure Function and Engineering group of Prof Joel Mackay (University of Sydney). In 2018 André started as a PhD candidate at the department of Physical Organic Chemistry of Prof Wilhelm Huck, in the coacervate and soft interfaces group of Dr Evan Spruijt in unravelling the mechanism and function of protein liquid-liquid phase separation by designing what we call artificial organelles.
- André, A.A.M., Yewdall, N.A., Spruijt, E., (2022) Crowding-induced phase separation and solidification by co-condensation of PEG in NPM1-rRNA condensates. BioRxiv (pre-print) Full text
- André*, A.A.M., Spruijt, E. (2020). Liquid-liquid phase separation in crowded environments, Int. J. Mol. Sci. 21(16), 5908 doi: 10.3390/ijms21165908 Full text
- Nakashima, K.K., Haren van, M.H.I., André*, A.A.M., Robu, I. & Spruijt, E. (2021). Active Coacervate droplets are protocells that grow and resist Ostwald ripening. Nat. Comm. 12(1), 1-11. doi:10.1038/s41467-021-24111-x Full text
- Yewdall, N.A., André*, A.A.M., Haren van, M.H.I., Nelissen, F.H.T., Jonker, A., Spruijt, E. (2021). ATP:Mg2+ shapes condensate properties of rRNA-NPM1 in vitro nucleolus model and itrs partitioning of ribosomes, BioRxiv, doi: 10.1101/2021.12.22.473778 Full text
- André*, A.A.M., & Spruijt, E. (2018). Rigidity rules in DNA droplets: Nucleic Acid Flexibility Affects Model Membraneless Organelles. Biophys. J. 115, 1-4. doi: 10.1016/j.bpj.2018.09.023 Full text