We combine state-of-the-art experimental genetics with complexome profiling and advanced imaging techniques to study the process of de novo cristae formation in malaria parasite mitochondria
The inner membrane of mitochondria, the powerhouses of the cell, is characterized by invaginations, termed cristae. Malaria parasites harbour a single, unusual mitochondrion that lacks cristae during the pathogenic blood-infection. After formation of sexual-stage parasites, required for transmission to the mosquito vector, mitochondrial functioning changes dramatically and cristae are formed from scratch. The investigators will employ live- and electron microscopy to provide an unparalleled 4D-reconstruction of cristae formation in this unique biological system. By combining these insights with data on protein complexes, they will shed light on this validated antimalarial drug-target and on basic principles of cristae formation.