Ion Chemistry / mass spectometry
Structural characterization of MS/MS product ions from protonated peptides
The development of infrared ion spectroscopy has revolutionized ion chemistry, in the sense that a reliable and accurate characterization of ion structures resulting from MS/MS reactions has become possible, often based on a comparison between measured ion spectra with DFT computed ones. We have been involved particularly in the characterization of MS/MS product ions of peptides, both from collision-induced dissociation (CID) as well as electron-transfer dissociation (ETD) reactions. For more information and articles on this topic, click here.
The ETD option of the Bruker instrument allows us to study transition metal ligand complexes in different redox states. We can generate and isolate M2+-ligand complexes, which can then be charge-reduced via electron transfer from the anionic ETD-reagent forming the corresponding M1+-ligand complex. Either the 1+ or the 2+ complex can be isolated in the ion trap and IR spectra can be recorded for each of the redox pairs. For more information and articles on this topic, click here.