Theme 2 colloquium: "IR photons as structural probe in mass spectrometry: the onset of peptide aggregation" (Lecture)
- Tuesday 13 February 2018Add to my calendar
- from 16:00
dr. Anouk Rijs (FELIX)
Mass spectrometry is a vital tool for the identification and quantification of proteins and their non-covalent assemblies. However, despite its remarkable sensitivity, the structural resolution is limited. In contract, optical -and especially infrared- spectroscopy provides a diagnostic probe of molecular structure. Interfacing IR action spectroscopy to mass spectrometry has enabled us to add an extra dimension to mass spectrometric analysis, providing structural characterization of lowly populated intermediates in heterogenic mixtures. This is extremely beneficial when probing the conformational changes in peptide aggregation and the molecular mechanism of (pathologic) amyloid formation, the self-assembly of soluble monomeric peptides into ordered structures. As nowadays the general consensus is that the actual malefactors of neurodegenerative diseases are not the full-grown fibrils, but the early-stage formed pre-fibrillar aggregates, it is key to determine the structure of these transient species. Our experiments aim to bring insights in the mechanism of peptide aggregation in a step-by-step fashion. In this talk, we will (1) explore the most informative infrared region for structural probing of the formation of peptide aggregates, (2) discuss the ideal experiment for our quest on the mechanism of peptide aggregation by adding a third dimension, and (3) show how we are using these advanced IR based MS methods for early and conclusive diagnostics of biomarkers of Parkinson's Disease.
dr. Evan Spruijt/dr. Peter Korevaar