IRP seminar by Cees Dekker (TU Delft): 'Employing nanotechnology for single-molecule biology: from protein sequencing to chromosome organization' (Lecture)
- Wednesday 16 November 2022Add to my calendar
- from 16:00
Nanotechnology offers fantastic opportunities to contribute to biology. I will present some recent examples from my lab where nanotech single-molecule tools are used to unravel the biology of cells down to the single-molecule level.
1. A DNA origami turbine powered by nanoscale flow 
We recently built an artificial nanoscale turbine. We demonstrated driven rotary motion of a nanoscale DNA origami turbine which harnessed energy from a water flow generated by a static chemical or electrical potential gradient in a solid-state nanopore. The origami nanoturbine consisted of a 6-helix DNA bundle that adopted a chiral conformation upon phoretic docking onto the nanopore and subsequently displayed a sustained unidirectional rotary motion of up to 20 revolutions/s. These artificial nano-engines operate autonomously in physiological conditions, converting energy into useful mechanical work.
2. Nanopore-based sequential reading of peptides 
We recently demonstrated a nanopore-based single-molecule peptide reader capable of reliably detecting single amino-acid substitutions within individual peptides. A peptide is linked to a DNA molecule and sequentially pulled through a biological nanopore by a DNA helicase in single amino-acid steps. Stepping ion-current signals enable discrimination of single-amino-acid substitutions in single reads. Notably, we demonstrated the capability to ‘rewind’ peptide reads, obtaining indefinitely many independent reads of the same molecule, yielding an undetectably low error rate in single-amino-acid variant identification. These proof-of-concept experiments constitute a promising basis for the development of a single-molecule protein sequencer.
3. Real-time imaging of DNA loop extrusion by condensin and cohesin SMC complexes 
Structural Maintenance of Chromosomes (SMC) proteins like cohesin and condensin spatially organize chromosomes by extruding DNA into large loops. Using single-molecule assays, we provided unambiguous evidence for loop extrusion by directly visualizing the processive extension of DNA loops by SMCs in real-time. In recent extensions of this work, we showed that how this process occurs on supercoiled DNA, how SMCs also can exhibit phase condensation, and that SMC proteins can bypass huge roadblocks of bound proteins on DNA.
 X. Shi et al, Nature Physics 18, 1105 (2022)
 H. Brinkerhoff et al, Science 374, 1509 (2021).
 Ganji et al, Science 360, 102 (2018); Kim et al, Nature 579, 438 (2020); B. Pradhan, BioRxiv, Cell Reports 19-10-2022.