Molecular biology 1: Advanced molecular tools
Course infoSchedule
Course moduleMED-BMS35
Credits (ECTS)3
CategoryMA (Master)
Language of instructionEnglish
Offered byRadboud University; Faculty of Medical Sciences; Biomedische wetenschappen;
Contactperson for the course
dr. K.A. Wolf
Other course modules lecturer
dr. K.A. Wolf
Other course modules lecturer
Academic year2017
1  (04/09/2017 to 26/08/2018)
Starting block
Course mode
RemarksPeriod 1a, Monday and Tuesday
Registration using OSIRISNo
Course open to students from other facultiesYes
Pre-registration openfrom 01/04/2017 up to and including 07/08/2017
Waiting listYes
Placement procedureDone manually by Back Office
ExplanationDone manually by Back Office
The main objectives of this module are:
After completion of the course, students are able to
  1. define the basics of the key genetic tools and advanced microscopy techniques available to design and create molecular probes for visualization of protein localization and function in a living cell.
  2. explain how complex molecular probes and advanced microscopy techniques can help to better understand the localization and function of a protein.
  3. perform transfection experiments using DNA constructs followed by basic and advanced microscopy as well as basic image analysis
  4. design an experimental strategy to study the function of a specific protein using tailored molecular probes and microscopy.
  5. evaluate experimental results and interpret them in a relevant cell biological context.
The module 

This course focuses on how to design and use genetically encoded molecular probes, with particular emphasis on probes that are used to study protein localization and function in living cells using basic and advanced microscopy techniques. The main questions that will be addressed are on how to design vectors, how to setup and perform complex live-imaging experiments, how to acquire and analyze microscopy images and how to interpret the results with respect to protein and cell function. The course aims to provide a broad overview of the experimental pipelines for designing and generating vectors encoding fusion proteins, cell transfections, microscopy techniques to monitor protein distribution, functional live cell imaging, and image analysis. The course further aims to refresh and extend the basics of cell adhesion, migration and mechanobiology. The course will consist of both a theoretical part (presentation, self-learning exercises, report) and a practical part.

Instructional modes
Working group

Period 1a, Monday and Tuesday

Course examination
Test weight1
OpportunitiesBlock 1, Block 1