Quantitative Bioimaging in Health and Disease
Course infoSchedule
Course moduleNWI-BB089
Credits (ECTS)6
Category02 (Introductory with prior knowledge)
Language of instructionEnglish
Offered byRadboud University; Faculty of Science; BioWetenschappen;
PreviousNext 1
prof. dr. A. Cambi
Other course modules lecturer
dr. K. van den Dries
Other course modules lecturer
dr. K. van den Dries
Other course modules lecturer
dr. K. van den Dries
Other course modules lecturer
dr. W.J.A.J. Hendriks
Other course modules lecturer
Academic year2021
KW3  (31/01/2022 to 10/04/2022)
Starting block
Course mode
RemarksMaximum capacity: 30. 2nd year Bachelor Biology students have priority
Registration using OSIRISYes
Course open to students from other facultiesNo
Waiting listYes
Placement procedureIn order of application
ExplanationIn order of application
After completion of the course, students are able to
  1. know the fundamentals of the major types of imaging modalities available
  2. understand the applications of different imaging techniques, both clinical and preclinical
  3. perform laboratory experiments to prepare and record fluorescence microscopic images
  4. gain hands-on experience with quantifying image information
  5. effectively communicate scientific information orally and in writing
How can we follow the movement of single receptors on the plasma membrane of cells? How can we visualize the growth and metastasis of tumors? How can we observe the interactions between antigen presenting cells and T-cells? Biomaging in the life sciences allows us to visualize these biological processes related to health and disease and is essential for research, prognosis and diagnosis. The field of bioimaging is rapidly evolving with many recent technological developments thereby further increasing our capabilities to address important biomedical questions.
This elective course will provide an overview of the various imaging modalities used in the laboratory. Going from molecules to tissues, students will learn about several key imaging techniques at a basic level (fluorescence and optical microscopy, live-cell imaging, electron microscopy, immunohistochemistry and intravital imaging). Students will also gain hands-on experience with some basic techniques that allow the qualitative and quantitative analysis of the different imaging modalities.
At the end of the course, students will have acquired an understanding of the advantages and disadvantages of each imaging modality and have embarked upon the rapidly developing roads towards the quantitative interpretation of the obtained images. As such, the course is a perfect preparation for imaging-based biological and biomedical research-oriented internships.

Instructional modes
  • (Interactive) Lecture
  • Presentation
  • Group work
  • individual Readiness Assurance Tests
  • Self-study

Presumed foreknowledge
This course has a strongly cell biological character. It is advised to refresh knowledge obtained in the courses Molecular Biology and Recombinant DNA (Q2 – BP010C), Cellular biochemistry (Q2 – BP007C), Histology (Q3 – BP006B) and Mathematics for Biologists (Q4 – BP024C). Students that want to refresh their knowledge in molecular and cellular biology may consult Alberts (4th edition), Lodish (8th edition) or Lehninger (6th edition).
Test information
Assessment consists of four parts:
  • Performance, initiative, input and participation during the Journal Club, group, counts for 10%, minimum grade 5.5
  • Written scientific report on image acquisition and analysis, group, counts for 20%, minimum grade 5.5
  • Midterm exam around week 5, individual, counts for 30%, minimum grade 5.5
  • Essay exam in week 10, individual, counts for 40%, minimum grade 5.5
The number of participants is maximized: everyone starts on the waiting list, and the education center will enrol the students to the actual course based on their programme (2nd year Ba-Bio students have priority) and on time of enrolment for the other students. This cannot be done before the enrolment deadline for the course has ended, so please make sure to enrol for a back-up course in time!
Required materials
Learning Management System (Brigthspace)
Assignments and literature references, the main materials for this course, will be provided on Brightspace.

Recommended materials
Standard textbooks on Cell Biology and Biochemistry (Lehninger, Alberts and/or Lodish).

Instructional modes
Group work
Attendance MandatoryYes

9 hours

iRAT (computer quiz)
Attendance MandatoryYes

6 hours

Journal Club (incl. presentation)
Attendance MandatoryYes

8 hours

Lectures & Interactive lectures
Attendance MandatoryYes

27 hours

Practical computer training
Attendance MandatoryYes

8 hours

Self -study
Attendance MandatoryYes

70 hours

Final Exam
Test weight4
Test typeExam
OpportunitiesBlock KW3, Block KW4

Midterm Exam
Test weight3
Test typeExam
OpportunitiesBlock KW3, Block KW3

Scientific Report
Test weight2
Test typeReport
OpportunitiesBlock KW3

Journal Club
Test weight1
Test typeAssignment
OpportunitiesBlock KW3