NWI-BP007C
Cellular Biochemistry
Course infoSchedule
Course moduleNWI-BP007C
Credits (ECTS)6
Category-
Language of instructionEnglish
Offered byRadboud University; Faculty of Science; BioWetenschappen;
Lecturer(s)
PreviousNext 5
Lecturer
prof. dr. A. Cambi
Other course modules lecturer
Lecturer
dr. M.H.A.G. Gorissen
Other course modules lecturer
Coordinator
dr. J.H.A. Martens
Other course modules lecturer
Lecturer
dr. J.H.A. Martens
Other course modules lecturer
Lecturer
dr. C.E.E.M. van der Zee
Other course modules lecturer
Academic year2018
Period
KW2  (05/11/2018 to 27/01/2019)
Starting block
KW2
Course mode
full-time
Remarks-
Registration using OSIRISYes
Course open to students from other facultiesYes
Pre-registrationNo
Waiting listNo
Placement procedure-
Aims
Course objectives
This course is to give the first-year bachelor students an introduction of basic concepts of biochemistry and cell Biology in animal cells
 
Learning goals
Upon completing this course, the student is able to:
  1. Understand and analyse how biological macromolecules in the cell are constructed and how they function (End qualification term P1).
  2. Understand how certain nutrients are taken up and via which metabolic pathway these nutrients are degraded for energy gain (End qualification term P1).
  3. Understand how the above processes functionally interact and how they are involved in human diseases, particularly the onset and progression of cancer (End qualification term P1).
  4. Apply the molecular and cell biological knowledge to the extent required to describe the physiological function of molecular components and regulators of the cell biological processes mentioned above (End qualification term P1).
  5. Work in a team, able to divide the tasks amongst the group and share gained results with the group (End qualification A3).
  6. Report their performed research in the form of a scientific report. (End qualification terms P7 en A4).
 
Specific learning goals for the content of the course
Upon completing this course, the student is able to:
  1. Understand and describe the primary, secondary, tertiary and quaternary structure of proteins based on fundamental chemical bonds mediating life.
  2. Describe the principles of experimental methods used to determine experimentally properties of proteins such as molecular mass or isoelectric point and methods used to separate and purify proteins.
  3. Analyze the most important parameters for enzyme kinetics and the role of different types of inhibitors.
  4. Understand and describe the properties fatty acids, cholesterol and phospholipids and their role in the composition and behavior of cellular membranes.
  5. Understand and describe how sugar structures originate and give rise to functional polymers.
  6. Understand the role Gibbs energy plays in chemical reactions and based on that indicate whether single or coupled chemical reactions can proceed spontaneously.
  7. Understand and describe key aspects of cell communication and cell signal transduction through membrane receptors and secondary messengers
  8. Understand and describe key aspects of regulation and function of the cytoskeleton in cell morphology, intracellular transport, cell migration and mitosis
  9. Understand and describe key aspects of integration of cells in tissues, and the functions of cell adhesion and extracellular matrix proteins
  10. Understand and describe basic concepts of cell cycle regulation including processes during the various stages of the cell cycle, how to identify cells at certain phases of the cell cycle and how polypeptide growth factors can control the cell cycle.
  11. Understand the evolutionary differences between cellular proto-oncogenes, activated cellular oncogenes and viral oncogenes, how activated cellular oncogenes and viral oncogenes give normal cells an autonomous growth behavior and how dominant activating mutations in cellular proto-oncogenes may lead to tumour growth.
  12. Understand the differences between normal cells, tumour cells and stem cells.

Course module 'Cytology' (0 EC) is part of the courses in this quarter: 
After completion of the Versatest module ‘Cytology’ you will have refreshed your memory, and high-school knowledge, of the animal cell. You can recognise, name, and explain the function of cell organelles and of key cellular processes.

 
Content
Topics Cellular biochemistry
  • Interactions in aqueous systems
  • Amino acids
  • Protein structure, folding and processing of proteins
  • Enzymes: working mechanisms, kinetics and inhibitors
  • Protein detection and purification
  • Catabolism of amino acids and fatty acids/lipids
  • Carbohydrates & Glycoconjugates
  • Principles of bioenergetics
  • Receptors and signal transduction
  • Cytoskeleton
  • Cell adhesion, extracellular matrix, epithelial mesenchymal transition
  • Cell cycle regulation, tumor suppressor genes, and oncogenes
  • Normal cells, stem cells and tumor cells
Content and Topics Course module 'Cytology'
Content

The Versatest module ‘Cytology’ prepares you for the courses in Q2 and Q3, among others ‘Histology’ (BP006B). We will address morphology and functions of key cellular components incl. the cytoplasm, cell membrane, nucleus, mitochondrion, ribosome, Golgi-apparatus, Rough and Smooth Endoplasmic Reticulum (RER/SER), Lysosome, Peroxisome, Cytoskeleton, Microtubules, Cilia, Centriole, (Micro)filament. Key cellular processes incl. active and passive transport, endo- and exocytosis, hetero- and autophagy, are also addressed.
The Versatest module ‘Cytology’ will be introduced in a short lecture (~30 min), after which you can follow the module online. A formative test is included, and can be completed in your own time.

Topics
Cytology of cytoplasm, cell membrane, nucleus, mitochondrion, ribosome, Golgi-apparatus, rough and smooth Endoplasmic Reticulum, Lysosome, Peroxisome, Cytoskeleton, Microtubules, Cilia, Centriole, (Micro)filament.

 
Additional comments
Additional comments Cellular Biochemistry
Students are asked to attend compulsory learning activities: (content) tutorials, the computer practical and the lab practical and related feedback sessions on the report. All learning activities are complementary to lectures, because not all course content can be elaborated in the lectures! Tutorials and the computer practical rehearse lectures and give you a chance to practice using the content. The lab practical gives you an opportunity to execute experiments using the content of one of the topics and to understand the topic in depth. All these learning activities together synergize to cover the matter you need to learn for this course.

The course is based on two books:

1. “Lehninger Principles of Biochemistry” (7th edition), Authors, David L Nelson, Michael Cox, ISBN 9781319150877
2. “Molecular Cell Biology” (8th Edition), Authors: Arnold Berk, Chris A. Kaiser, Harvey Lodish, Angelika Amon, Hidde Ploegh, Anthony Bretscher, Monty Krieger, Kelsey C. Martin, ISBN 9781464187445


The assignments for the tutorials, computer labs and lab courses can be found in the manual (available via Brightspace). Please note that the files in Brightspace will be updated whenever new information is available to students. The Powerpoint slides of the lectures can be downloaded in pdf format via Blackboard during the course.

The lectures this academic year will be recorded on video. You will find the recordings on Brightspace, in the folder “Weblectures”.

Required Materials for the course module Cytology (0 EC):

Mescher (A.L.): Junqueira’s Basic Histology, Text and Atlas, 14th or 15th ed. This book is also intensively used in the ‘Histology’ course (BP006B).

Instructional modes for Cytology course
~30 min introductory lecture
~1 h 15 min Versatest (computer) practical

Test information
The final grade consists of 80% the exam score and 20% the lab practical score and must be 5.5 to pass the course. Please note that a minimum score of 5.5 for both the written exam and the lab practical is needed to pass the exam.

Course module Cytology:
The Versatest module ‘Cytology’ will be introduced in a short lecture (~30 min), after which you can follow the module online. A formative test is included, and can be completed in your own time. (Multiple Choice and point-and-click questions via Versatest).

Prerequisites
Dutch high school vwo-level, chemistry and biology knowledge required

Prerequisites course module Cytology:
High school level cytology and animal cell biology.

Required materials
Book
“Lehninger Principles of Biochemistry” (7th edition), Authors, David L Nelson, Michael Cox, ISBN 9781319150877
ISBN:9781319150877
Title:Lehninger Principles of Biochemistry
Author:David L Nelson, Michael Cox
Course guide
Course guidelines
Course guide
lecture slides, explanations about the lab courses and computer practical
Book
“Molecular Cell Biology” (8th Edition), Authors: Arnold Berk, Chris A. Kaiser, Harvey Lodish, Angelika Amon, Hidde Ploegh, Anthony Bretscher, Monty Krieger, Kelsey C. Martin, ISBN 9781464187445
ISBN:9781464187445
Title:Molecular Cell Biology
Author:Arnold Berk, Chris A. Kaiser, Harvey Lodish, Angelika Amon, Hidde Ploegh, Anthony Bretscher, Monty Krieger, Kelsey C. Martin

Instructional modes
Course occurrence

Lab course
Attendance MandatoryYes

Lecture

Response course

Tutorial
Attendance MandatoryYes

Zelfstudie

Tests
Exam
Test weight1
Test typeExam
OpportunitiesBlock KW2, Block KW3