After this course, you are able to:
· Plan, understand and correctly carry out a (multistep) synthetic protocol and use appropriate purification methods to obtain molecules that can be used to manipulate a specific biomolecule.
· Analyze and correctly interpret analytical data for the synthesized molecules with spectroscopic techniques such as TLC, NMR, IR, LC-MS, UV-Vis and fluorescence.
· Evaluate and correctly interpret results for the synthesized molecules on a specific biomolecule with the use of appropriate biochemical techniques.
· Document the set-up of the experiment and the results in a scientific and adequate manner in a lab journal and present the results in a research report.
The relatively new research discipline of chemical biology aims at developing (bioorthogonal) chemical methods and molecular tools, such as biosensors or fluorescent molecules, that can be used to study complex biological systems. In this practical course you will extend your knowledge, skills and advanced concepts in synthetic organic chemistry and apply the obtained molecules to study proteins and other biomolecules that are present in cells. Although this course contains aspects of both synthetic organic chemistry as well as biochemistry, the focus lies on acquiring and expanding the chemistry knowledge and skills.