NWI-MOL078
Nanobiotechnology
Course infoSchedule
Course moduleNWI-MOL078
Credits (ECTS)6
CategoryBA (Bachelor)
Language of instructionEnglish
Offered byRadboud University; Faculty of Science; Moleculaire Wetenschappen;
Lecturer(s)
Coordinator
prof. dr. R.E. Brock
Other course modules lecturer
Lecturer
prof. dr. R.E. Brock
Other course modules lecturer
Contactperson for the course
prof. dr. R.E. Brock
Other course modules lecturer
Lecturer
prof. dr. D.A. Wilson
Other course modules lecturer
Academic year2017
Period
KW2  (13/11/2017 to 04/02/2018)
Starting block
KW2
Course mode
full-time
Remarks-
Registration using OSIRISYes
Course open to students from other facultiesYes
Pre-registrationNo
Waiting listNo
Placement procedure-
Aims

At the end of the course the student is able to:

  • appreciate nanobiotechnology as a rapidly developing area of research that aims at creating functional units at the nanoscale for use in biomedicine, diagnostics and biotechnology.
  • appreciate dimensions in molecular cell biology and understands how tools in nanobiotechnology match these dimensions and can be used to obtain a deeper understanding of molecular processes.
  • apply knowledge on conjugation, micromanipulation and self-organization strategies to create targeted molecular sensors and drug delivery vehicles in biomedicine.
  • select the appropriate microscopic technique to analyse nanostructures in nanobiotechnology based on a fundamental understanding of the techniques.
  • appreciate how the integration of microfluidics and single-molecule detection technologies enables the design of powerful microanalytical instruments.
  • critically read and present a scientific article in relation to its context.
Content
The course will cover various aspects of applications of nanobiotechnology in drug delivery and diagnostics, analytical methods and microsystems for control, manipulation and analysis. The ten-week 6 EC course is structured into four thematic blocks with lectures, each with 2 h guided selfstudy (werkcollege) and 1 h of discussion (responsie-college). Thematic blocks are chemistry for nanobiotechnology, imaging (visualization of molecules and structures at the nanoscale), manipulation and analysis of molecules at the nanoscale and nanodevices/drug delivery systems.
Along the course the students will participate in various practical demonstrations and hands-on experiments. In addition, students – in groups of three - will be assigned a subject. Students will write a script about this topic and give a presentation at the end of the course. The course will conclude with an exam.
Literature
Literature will be made available via Blackboard in the form of publications, copies from textbooks and Power Point presentations. In addition, there will be video recordings on all lectures.
Furthermore, the following two texts “Nanomedicine: Nanotechnology for Health” and “European Technology Platform on NanoMedicine” which can be down-loaded via http://cordis.europa.eu/nanotechnology/nanomedicine.htm are recommended as an introduction.

Teaching formats
• 24 hours lecture
• 16 hours laboratory course
• 6 hours question session
• 8 hours problem session

Topics
The course will cover:

1) Chemistry for nanobiotechnology
• functional groups in biomolecules
• methods for a directed immobilization and manipulation of molecules

2) Visualization of biomolecules at the nanoscale
• probes for nanobiotechnology
• (superresolution) imaging

3) Manipulation and analysis of biomolecules at the nanoscale
• molecular and physical microstructuring of surfaces
• single-molecule detection and analysis (scanning probe and fluorescence based techniques)

4) Nanodevices/Drug delivery
• liposome-based drug delivery
• functionalized nanoparticles/quantum dots for targeted labeling
• nano-/microdelivery systems

Experimental techniques for practical demonstrations/ experiments by the students
Experiments will be in part demonstrations and in part hands-on, also depending on the number of students enrolled in the course.
• generation of polymersomes
• size measurements of nanoparticles using dynamic light scattering (DLS)
• intracellular trafficking of polyplexes
• Surface probe microscopy

Test information
At the end of the course there will be a written exam. The result of the exam will contribute 50 % to the final grade. The script will contribute another 25 %, the presentation another 25 %. For each part at least 5.0 has to be obtained to gain credits for the course. If this requirement is not met in any of the parts, only the respective part will have to be repeated.

Prerequisites
This course is primarily directed towards MLW students. Good fundamental knowledge of chemistry and physics is therefore required. This is a course in the programme 'Molecular Life Sciences' and in the minor 'bio-organic chemistry'

For students in the minor: prerequisite is NWI-MOL101 Essentials of molecular chemistry.

Required materials
Blackboard
Literature will be made available via Blackboard in the form of publications, copies from textbooks and Power Point presentations.

Recommended materials
Articles
The following two texts “Nanomedicine: Nanotechnology for Health” and “European Technology Platform on NanoMedicine” which can be down-loaded via http://cordis.europa.eu/nanotechnology/nanomedicine.htm are recommended as an introduction.

Instructional modes
Lab course
Attendance MandatoryYes

Remark
Maximum number of participants: 100

Lecture

Remark
Maximum number of participants: 100

Response course

Remark
Maximum number of participants: 100

Tutorial

Tests
Tentamen
Test weight1
OpportunitiesBlock KW2, Block KW4