At the end of the course, the student is able to:
- explain the relevance of data processing methods for 1D and 2D datasets and images in chemical analysis.
- evaluate the data from Fourier based analytical methods (FT-IR, NMR, MRI, FT-MS (ICR) and crystallography).
- define acquisition parameters of the main Fourier-based methodologies in chemical analysis.
- evaluate appropriate methods to phase correct and filter raw spectroscopic data and calculate their Fourier transform.
- list a set of functions occurring frequently in physical sciences and their Fourier transforms.
- explain and apply the basic mathematical principles of Fourier analysis and the related Fourier theorems.
- explain which microscopic technique is the most suitable to image or measure a certain object.
- recognize different microscopic techniques based on their images.
- explain and apply the principles and operation of the most frequently used microscopic techniques (classical optical microscopy, phase sensitive optical microscopy, interferometry, fluorescence, polarized light and superresolution microscopy, electron microscopy, and scanning probe microscopy), and to determine their possibilities and limitations.
- develop and apply elementary image processing methods to improve microscopic images and retrieve information from them.
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Chemists rely on various analytical technologies using specific instrumentation for characterizing samples of any kind either qualitatively or quantitatively. These technologies yield data in the form of spectra or images, which often require further processing or filtering to extract the relevant information.
Fourier analysis is of great relevance in many applications in natural sciences, as many phenomena and processes are inherently periodic. The Fourier transform is one of the most used mathematical tools for the analysis and interpretation of periodic signals. This course introduces Fourier series and gives an overview of the theory underlying the Fourier transform (FT) and its use. The power of this approach will be demonstrated by discussing the application of the FT in Spectroscopy, Imaging and X-ray diffraction. During the computer labs processing of NMR, IR and MS data will be practised using the Matlab computing environment.
Microscopy is a collection of techniques aimed at visualizing objects that cannot be seen by the naked eye. Microscopic techniques yield images, which are essentially 2D datasets or signals. This course introduces the most commonly used microscopic techniques, including classical optical microscopy, phase sensitive microscopy, interferometry, fluorescence microscopy, polarized light microscopy and superresolution microscopy, transmission and scanning electron microscopy, atomic force microscopy and scanning tunneling microscopy. By treating the light or electrons either as rays or waves, both classical optics and Fourier methods can be used to visualize and measure objects. During the computer lab, image processing methods will be practiced using Matlab.
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Written exam (60%) and report computer lab (40%). Both elements must be completed with a grade of at least 5.0.
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