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NMR facility

The NMR facility is suitable for nondestructively characterizing the structure and dynamic processes (i.e. binding, kinetics, diffusion, chemical exchange, etc) of soluble chemical, biological and material compounds. Users routinely acquire spectra on samples ranging from small molecules (<200 Da) to polymers (>2 kDa) and up to massive macromolecules (~2 MDa). The facility currently consists of three instruments:NMR

A) Agilent Inova 400 MHz:
HX Inverse probe: 1H, 13C; room temperature experiments.

B) Bruker Avance III 400 MHz (with 120 sample autosampler):
BBFO probe: 1H, 19F on X, 31P, 13C, 15N, nuclei down to 109Ag in freq.; - 130 °C - + 130 °C.

C) Bruker Avance III 500 MHz (with 120 sample autosampler):
Prodigy cryoprobe: 1H, 19F on 1H, 31P, 13C, nuclei down to 15N in freq.; -20 °C - +60 °C.
BBFO probe:  1H, 19F on X, 31P, 13C, 15N, nuclei down to 109Ag in freq.; - 130 °C - + 130 °C.

The Inova is frequently used for fast, routine acquisition of 1H spectra while the Bruker instruments are used for full structural characterization, variable-temperature, kinetics and 2D experiments such as COSY, HSQC, HMBC, NOESY and diffusion (DOSY). The presence of an autosampler on each Bruker instrument allows users to perform many of these experiments automatically overnight.

Additionally, the facility received a Prodigy cryoprobe for the Bruker 500 MHz, which boosts the sensitivity of the instrument by a factor of 2-3. This allows users to acquire routine spectra with 6-10 times fewer scans or use 2-3 times less material in their sample. The probe has greatly reduced the time to acquire traditionally time-consuming experiments such as 13C to often times under 30 minutes as well as expanded our ability to measure publishable data at low concentration (~100 μM) routinely.

NMR spectroscopy is a wide and highly applicable field to a large range of scientists, and collaborations to perform experiments that are not currently ready in the facility are welcomed.

For whom?

For researchers and companies who need accurate structure elucidation of their chemical compounds or wish to investigate dynamic processes.

Contact

Dr. Paul White
T: +31 24 36 52959
E: p.white@science.ru.nl

More information about this facility can be found here. You can also contact the central coordination of Radboud Research Facilities.
E: info@radboudresearchfacilities.nl