JEOL 6330 Cryo FESEM
This JEOL Field Emission Sacnning Electron Microscope can be used either in conventional mode or as cryo-SEM. There is a separate entry chamber for each technique.
- In conventional use, the material needs to be naturally conductive or it has to be precoated with a thin layer of metal (see SEM coaters).
- In cryo-mode, the material is first fixed with nitrogen slush (and tests are going on to apply high-pressure freezing) and transposed to the cryo-chamber.
- When observation of the inner structure of the object is seeked, the frozen material is then cleaved with a tiny knife. Still in this same preparation chamber under vacuum a sublimation step is performed and coating with gold-palladium (more information and photographs about the preparation procedure...).
- There are a number of different holders: on some holders the sample can be tilted and there are also possibilities to make stereo-views (always ask assistance first) .