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Image Gallery

If you have cool images acquired at the GI and you would like to share them, please send an email to Jelle Postma j.postma@science.ru.nl

Multiview light sheet data of an Arabidopsis thaliana mature anther with pollen grains, stained with Alexander's staining protocol. Multiview images were taken with an Olympus/PhaseView Alpha3 Light Sheet system. Image was visualized with the Huygens MIP Renderer using depth-code colouring. General Instrumentation Department © 2019

Light Sheet image of an Arabidopsis thaliana root endogenously expressing LTi6 plasma membrane marker which marks all cells (orange) and DR5 the auxin plant hormone marker, present in Quiescent centre (QC) and columella root cap cells (green). Imaged with PhaseView Alpha3 system. General Instrumentation Department © 2019

Static Oligodendrocyte Precursor Cell Stationary Oligodendrocyte Precursor Cell (OPC) at the dorsal part of a 3dpf zebrafish spinal cord, expressing Nkx2.2a-eGFP. Most of the vertebrates’ CNS axons are wrapped by myelin produced by oligodendrocytes. During development the migrating OPCs extend long membrane processes avoiding neighboring OPCs and identifying the region that will be immobilized. The OPCs membrane extensions become ramified during axonal identification. The acquired confocal z-stack was deconvolved using Huygens deconvolution software and then depth color-coded using ImageJ. Scale bar 5um. Image courtesy of Dr. I. Alexopoulos

Maximum intensity projection of a confocal acquisition of a neuromuscular junction from mouse plantaris muscle. The neuronal part of the junction is shown in magenta marking the synaptic vesicle glycolprotein 2A (SVA2) and the neurofilaments, while in yellow is shown the acetylcholine receptors on the muscular part (using bungarotoxin).

Interference Reflection Microscopy (IRM)(https://en.wikipedia.org/…/Interference_reflection_microsco…) using our Leica SP8-AOBS-WLL confocal microscope. An easy way to visualise and measure cell membrane proximity to substrate. The diagram shows how the high intensity of vinculin proteins co-localize with the low intensities of the IRM signal in focal adhesions of mesenchymal stem cells. Sample Preparation by Lina Chen

Combined reflections of 4 different wavelengths (594nm, 612nm, 620nm, 670nm) from sequential optical sections of a cleared mouse brain tissue, where neutrons are stain with mercury. Sample provided by Prof. Dr. Tansu Celikel and prepared by Tang Zhengyu.

Zebrafish spinal cord myelination Time-lapse imaging of the spinal cord development of a 2dpf transgenic zebrafish, expressing Nkx2.2a-eGFP (cyan) and Cntn1a-mCherry (magenta). The oligodendrocyte precursor cells (OPCs), start migrating dorsally and along the neuronal axons forming filopodia-like extensions. After finding the right position, their extensions become ramified and eventually start the ensheathment of neighboring axons. Dorsal is up and anterior on the right. Time interval 15min. Scale bar 10um. Image courtesy of Dr. I. Alexopoulos

Myelinating Oligodendrocytes Myelinating oligodendrocytes (OLs) at the dorsal part of a 3dpf zebrafish spinal cord, expressing Nkx2.2a-eGFP. The Nkx2.2a protein is present at a subset of oligodendrocyte precursors and OLs as well as in other non-related cells. Immature OLs initially start wrapping most of the vertebrates’ CNS axons while the production of myelin is followed. The acquired confocal z-stack was deconvolved using Huygens deconvolution software and then 3D reconstructed using Imaris/Bitplane. Scale bar 5um. Image courtesy of Dr. I. Alexopoulos

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