We offer various technologies for the investigation of glycosylation. These technologies are made available to researchers within the RCG, but also for external collaborations. Please contact us if you would like to include these thechologies in your research.
Mass spectrometry based analytical tools:
- Mass spectrometric analysis of purified glycoproteins or whole glycoproteome profiling from biological samples like bio fluids. These technologies can be used to characterize glycosylation profiles for research and diagnostic purposes.
- Calculator for mass prediction of glycans, glycopeptides and glycoproteins at glycomass.com
- N-glycan profiling from biological samples
- Flux-analysis of metabolites involved in carbohydrate metabolism such as nucleotide sugars and sugar phosphates.
We developed synthetic glycosylation modulators to track and perturb glycan synthesis and recognition. These tools can be used in cell experiments, bacteria or in animal models such as zebra fish and mice. The new compounds have been made available to the consortium members and collaborative projects have been initiated.
We offer advanced bioimaging techniques to visualize cellular defects due to aberrant glycosylation. These techniques include: super-resolution fluorescent microscopy, CryoEM, Correlative Light and Electron Microscopy (CLEM).
We established zebrafish models to study immune cell function in congenital disorders of glycosylation.